Fibonacci spiral is widely identified in non-biological fields such as mathematics, architecture and art, but not in human biology; an understanding of its functional role in human anatomy may be essential in making further advances in biomedical

engineering specifically knee implant design. This study aims to explore whether the Golden Ratio and Fibonacci Spiral approximate the shape of the distal femoral articular surface of the knee. Twenty-three 3D CT scan images of the distal knee were digitalized in 2D sagittal views and best fitted over with Fibonacci spirals computed from the knee antero-posterior dimensions. The differences between the means of the radii of the Fibonacci Spiral and the digitalized images on 5/6 subsectors where not significant at p<0.05 using Krus-Wallis Test covering 84% of the distal articulating surface. With the Fibonacci presenting three independent axes described a “L” shaped on the sagittal plane, these findings affirm the fixed, flexion-extension axes of knee kinematics.